Regulatory

Part:BBa_K619898:Design

Designed by: Julianne Grose   Group: iGEM11_BYU_Provo   (2011-09-28)


medium length prfA-UTR thermosensor from Lysteria Monocytogenes


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our "wild-type" thermosensor (BBa-19889) Shine-Delgarno has been optimized for expression in E. coli. Otherwise, the "wild-type" sequence is identical to the thermosensor sequence from Listeria monocytogenes.


Source

This is a truncated prfA-UTR from Listeria monocytogenes made by annealing primers with the desired sequence.

References